Optimization of Chelex 100 resin-based extraction of genomic DNA from dried blood spots
نویسندگان
چکیده
منابع مشابه
Genomic Dna Extraction from Dried Blood Spots
We have developed an efficient and simple method for extracting and purifying genomic DNA from dried blood stored on filter paper. The quality of the genomic DNA extracted is tested by PCR amplification of a 255-bp fragment of the PAX8 gene sequence and the PCR products are determined for further genetic studies by single strand conformation polymorphism (SSCP) analysis. Larger DNA sequences of...
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The presence of organic compounds and high amount of secondary metabolites (polysaccharides, phenolic component, etc.) in mosses cause difficulties in DNA extraction that are followed by problems in PCR reactions. In lower plants, various methods have been used for DNA extraction including silica gel and different commercial kits. These methods mostly use hazardous (like phenol or liquid nitrog...
متن کاملCost-effective and scalable DNA extraction method from dried blood spots.
BACKGROUND Dried blood spot (DBS) samples have been widely used in newborn screening (NBS) for the early identification of disease to facilitate the presymptomatic treatment of congenital diseases in newborns. As molecular genetics knowledge and technology progresses, there is an increased demand on NBS programs for molecular testing and a need to establish reliable, low-cost methods to perform...
متن کاملOptimized DNA extraction from neonatal dried blood spots: application in methylome profiling
BACKGROUND Neonatal dried blood spots (DBS) represent an inexpensive method for long-term biobanking worldwide and are considered gold mines for research for several human diseases, including those of metabolic, infectious, genetic and epigenetic origin. However, the utility of DBS is restricted by the limited amount and quality of extractable biomolecules (including DNA), especially for genome...
متن کاملRapid DNA Extraction from Dried Blood Spots on Filter Paper: Potential Applications in Biobanking
OBJECTIVES Dried blood spot (DBS) technology is a microsampling alternative to traditional plasma or serum sampling for pharmaco- or toxicokinetic evaluation. DBS technology has been applied to diagnostic screening in drug discovery, nonclinical, and clinical settings. We have developed an improved elution protocol involving boiling of blood spots dried on Whatman filter paper. METHODS The pu...
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ژورنال
عنوان ژورنال: Biology Methods and Protocols
سال: 2020
ISSN: 2396-8923
DOI: 10.1093/biomethods/bpaa009